![]() If a polydispersity index is less than 20%, then the molecular species is mono-disperse, or else it is heterogeneous. There was more HPC with a longer decay rate at pH 3.0 (up to ~1.0 x 10 4 µs) than that at pH 7.5 (up to ~2.0 x 10 2 µs). 1) Buffer at pH 3.0 and pH 7.5 (overlapped), 2) HPC at pH 7.5, and 3) HPC at pH 3.0. ![]() Data was collected with a 5 seconds acquisition time, 10 acquisitions per measurement at laser power 100% (buffer) and 90% (HPC samples). The auto correlation function for HPC at pH 3.0 and pH 7.5. The polydispersity index provides a direct indicator of the heterogeneity of the each molecular species in the sample.įigure 1. The size distribution of the sample can be directly measured from the intensity auto correlation function, as shown in Figure 1. The Wyatt DynaPro DLS system coupled with Dynamics ® V7 was used to acquire the DLS data at 20 ☌, and Dynamics ® V7.0.0.95 was used to perform the analysis. Observations revealed that the pH of the buffer solutions had an influence on the homogeneity and stability of the sample. According to electron microscopy observation, the total molecular weight is 65 kDa for HPC, which is susceptible to create fibril materials in vitro.Īn analysis was performed to characterize homogeneity and stability of HPC utilizing the Wyatt DynaPro ® dynamic light scattering (DLS) system under many different buffer conditions. Sponsored by Wyatt Technology Aug 16 2013Ī heterodimeric protein complex (HPC) derived from Pyrococcus furiosus is recognized for its functional role in RNA binding.
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